"Current Clamp" is a common technique in electrophysiology. This is a whole-cell current clamp recording of a neuron firing due to it being depolarized by current injection Electrophysiology is the study of the electrical properties of biological cells and tissues. It involves measurements of voltage change or electrical current flow on a wide variety of scales from single ion channel proteins to whole tissues like the heart. In neuroscience, it includes measurements of the electrical activity of neurons, and particularly action potential activity. Contenido 1 Definition and scope 1.1 Classical electrophysiological techniques 1.2 Optical electrophysiological techniques 2 Intracellular recording 2.1 Voltage clamp 2.2 Current clamp 2.3 The patch-clamp technique 2.4 Sharp electrode technique 3 Extracellular recording 3.1 Single Unit recording 3.2 Field potentials 3.3 Amperometry 4 Planar patch clamp 5 Ver también 6 References & Bibliography 7 Textos clave 7.1 Libros 7.2 Papeles 8 Material adicional 8.1 Libros 8.2 Papeles 9 Enlaces externos 9.1 External links for planar patch clamp Definition and scope Classical electrophysiological techniques Classical electrophysiology techniques involve placing electrodes into various preparations of biological tissue. The principal types of electrodes are: 1) simple solid conductors, such as discs and needles (singles or arrays), 2) tracings on printed circuit boards, y 3) hollow tubes filled with an electrolyte, such as glass pippettes. The principal preparations include 1) living organisms, 2) excised tissue (acute or cultured), 3) dissociated cells from excised tissue (acute or cultured), 4) artificially grown cells or tissues, o 5) hybrids of the above. If an electrode is small enough (micrometres) en diámetro, then the electrophysiologist may choose to insert the tip into a single cell. Such a configuration allows direct observation and recording of the intracellular electrical activity of a single cell. Sin embargo, at the same time such invasive setup reduces the life of the cell. Intracellular activity may also be observed using a specially formed (hollow) glass pipette. In this technique, the microscopic pipette tip is pressed against the cell membrane, to which it tightly adheres. The electrolyte within the pipette may be brought into fluid continuity with the cytoplasm by delivering a pulse of pressure to the electrolyte in order to rupture the small patch of membrane encircled by the pipette rim (whole cell recording). Alternativamente, ionic continuity may be established by "perforating" the patch by allowing exogenous ion channels within the electrolyte to insert themselves into the membrane patch (perforated patch recording). Finalmente, the patch may be left intact (patch recording). The electrophysiologist may choose not to insert the tip into a single cell. En lugar de, the electrode tip may be left in continuity with the extracellular space. If the tip is small enough, such a configuration may allow indirect observation and recording of the electrical activity of a single cell, and is termed single unit recording. Depending on the preparation and precise placement, an extracellular configuration may pick up the activity of several nearby cells simultaneously, and this is termed multi-unit recording. As electrode size increases, the resolving power decreases. Larger electrodes are sensitive only to the net activity of many cells, termed local field potentials. Still larger electrodes, such as uninsulated needles and surface electrodes used by clinical and surgical neurophysiologists, are sensitive only to certain types of synchronous activity within populations of cells numbering in the millions. Other classical electrophysiological techniques include single channel recording and amperometry. Optical electrophysiological techniques Optical electrophysiological techniques were created by scientists and engineers to overcome one of the main limitations of classical techniques. Classical techniques allow observation of electrical activity at approximately a single point within a volume of tissue. Essentially, classical techniques singularize a distributed phenomenon. Interest in the spatial distribution of bioelectric activity prompted development of molecules capable of emitting light in response to their electrical or chemical environment. Examples are voltage sensitive dyes and fluoresceing proteins. After introducing one or more such compounds into tissue via perfusion, injection or gene expression, el 1 or 2-dimensional distribution of electrical activity may be observed and recorded. Please help improve this article by expanding this section. See talk page for details. Elimine este mensaje una vez que se haya ampliado la sección.. Many particular electrophysiological readings have specific names: Electrocardiography - for the heart Electroencephalography - for the brain Electrocorticography - from the cerebral cortex Electromyography - for the muscles Electronystagmography Electroplethysmography Electrooculography - for the eyes Electroretinography - for the retina Electroantennography - for the olfactory receptors in arthropods Magnetoencephalography Polysomnography Intracellular recording Intracellular recording involves measuring voltage and/or current across the membrane of a cell. To make an intracellular recording, the tip of a fine (sharp) microelectrode must be inserted inside the cell, so that the membrane potential can be measured. Típicamente, the resting membrane potential of a healthy cell will be -60 Para -80 mV, and during an action potential the membrane potential might reach +40 mV. En 1963, Alan Lloyd Hodgkin and Andrew Fielding Huxley won the Nobel Prize in Physiology or Medicine for their contribution to understanding the mechanisms underlying the generation of action potentials in neurons. Their experiments involved intracellular recordings from the giant axon of Atlantic squid (Loligo pealei), and were among the first applications of the "abrazadera de voltaje" technique. Hoy, most microelectrodes used for intracellular recording are glass micropipettes, with a tip diameter of < 1 micrometre, and a resistance of several megaohms. The micropipettes are filled with a solution that has a similar ionic composition to the intracellular fluid of the cell. A chlorided silver wire inserted in to the pipet connects the electrolyte electrically to the amplifier and signal processing circuit. The voltage measured by the electrode is compared to the voltage of a reference electrode, usually a silver-silver chloride wire in contact with the extracellular fluid around the cell. In general, the smaller the electrode tip, the higher its electrical resistance, so an electrode is a compromise between size (small enough to penetrate a single cell with minimum damage to the cell) and resistance (low enough so that small neuronal signals can be discerned from thermal noise in the electrode tip). Voltage clamp The voltage clamp uses a negative feedback mechanism. The membrane potential amplifier measures membrane voltage and sends output to the feedback amplifier. The feedback amplifier subtracts the membrane voltage from the command voltage, which it receives from the signal generator. This signal is amplified and returned into the cell via the recording electrode. The voltage clamp technique allows an experimenter to "clamp" the cell potential at a chosen value. This makes it possible to measure how much ionic current crosses a cell's membrane at any given voltage. This is important because many of the ion channels in the membrane of a neuron are voltage gated ion channels, which open only when the membrane voltage is within a certain range. Voltage clamp measurements of current are made possible by the near-simultaneous digital subtraction of transient capacitive currents that pass as the recording electrode and cell membrane are charged to alter the cell's potential. (See main article on voltage clamp.) Current clamp The current clamp technique records the membrane potential by injecting current into a cell through the recording electrode. Unlike in the voltage clamp mode, where the membrane potential is held at a level determined by the experimenter, in "current clamp" mode the membrane potential is free to vary, and the amplifier records whatever voltage the cell generates on its own or as a result of stimulation. This technique is used to study how a cell responds when electrical current enters a cell; this is important for instance for understanding how neurons respond to neurotransmitters that act by opening membrane ion channels. Most current-clamp amplifiers provide little or no amplification of the voltage changes recorded from the cell. The "amplifier" is actually an electrometer, sometimes referred to as a "unity gain amplifier"; its main job is to change the nature of small signals (in the mV range) produced by cells so that they can be accurately recorded by low-impedance electronics. The amplifier increases the current behind the signal while decreasing the resistance over which that current passes. Consider this example based on Ohm's law: a voltage of 10 mV is generated by passing 10 nanoamperes of current across 1 MΩ of resistance. The electrometer changes this "high impedance signal" to a "low impedance signal" by using a voltage follower circuit. A voltage follower reads the voltage on the input (caused by a small current through a big resistor). It then instructs a parallel circuit that has a large current source behind it (the electrical mains) and adjusts the resistance of that parallel circuit to give the same output voltage, but across a lower resistance. The patch-clamp technique The cell-attached patch clamp uses a micropipette attached to the cell membrane to allow recording from a single ion channel. Main article: Patch clamp This technique was developed by Erwin Neher and Bert Sakmann who received the Nobel Prize in 1991. Conventional intracellular recording involves impaling a cell with a fine electrode; patch-clamp recording takes a different approach. A patch-clamp microelectrode is a micropipette with a relatively large tip diameter. The microelectrode is placed next to a cell, and gentle suction is applied through the microelectrode to draw a piece of the cell membrane (the 'patch') into the microelectrode tip; the glass tip forms a high resistance 'seal' with the cell membrane. This configuration is the "cell-attached" mode, and it can be used for studying the activity of the ion channels that are present in the patch of membrane. If more suction is now applied, the small patch of membrane in the electrode tip can be displaced, leaving the electrode sealed to the rest of the cell. This "whole-cell" mode allows very stable intracellular recording. A disadvantage (compared to conventional intracellular recording with sharp electrodes) is that the intracellular fluid of the cell mixes with the solution inside the recording electrode, and so some important components of the intracellular fluid can be diluted. A variant of this technique, the "perforated patch" technique, tries to minimise these problems. Instead of applying suction to displace the membrane patch from the electrode tip, it is also possible to withdraw the electrode from the cell, pulling the patch of membrane away from the rest of the cell. This approach enables the membrane properties of the patch to be analysed pharmacologically. Sharp electrode technique In situations where one wants to record the potential inside the cell membrane with minimal effect on the ionic constitution of the intracellular fluid a sharp electrode can be used. These micropipets (electrodes) are again like those for patch clamp pulled from glass capillaries, but the pore is much smaller so that there is very little ion exchange between the intracellular fluid and the electrlolyte in the pipete. The resistance of the electrode in 10s or 100s of MΩ in this case. Often the tip of the electrode is filled with various kinds of dyes like Lucifer yellow to fill the cells recorded from, for later confirmation of their morphology under a microscope. The dyes are injected by applying a positive or negative, DC or pulsed voltage to the electrodes depending on the polarity of the dye. Extracellular recording Single Unit recording Main article: single unit recording An electrode introduced into the brain of a living animal will detect electrical activity that is generated by the neurons adjacent to the electrode tip. If the electrode is a microelectrode, with a tip size of about 1 micrometre, the electrode will usually detect the activity of at most one neuron. Recording in this way is generally called "single unit" recording. The action potentials recorded are very like the action potentials that are recorded intracellularly, but the signals are very much smaller (typically about 1 mV). Most recordings of the activity of single neurons in anesthetized animals are made in this way, and all recordings of single neurons in conscious animals. Recordings of single neurons in living animals have provided important insights into how the brain processes information. For example, David Hubel and Torsten Wiesel recorded the activity of single neurons in the primary visual cortex of the anesthetized cat, and showed how single neurons in this area respond to very specific features of a visual stimulus. Hubel and Wiesel were awarded the Nobel Prize in Physiology or Medicine in 1981. If the electrode tip is slightly larger, then the electrode might record the activity generated by several neurons. This type of recording is often called "multi-unit recording", and is often used in conscious animals to record changes in the activity in a discrete brain area during normal activity. Recordings from one or more such electrodes which are closely spaced can be used to identify the number of cells around it as well as which of the spikes come from which cell. This process is called spike sorting and is suitable in areas where there are identified types of cells with well defined spike characteristics. If the electrode tip is bigger still, generally the activity of individual neurons cannot be distinguished but the electrode will still be able to record a field potential generated by the activity of many cells. Field potentials A schematic diagram showing a field potential recording from rat hippocampus. At the left is a schematic diagram of a presynaptic terminal and postsynaptic neuron. This is meant to represent a large population of synapses and neurons. When the synapse releases glutamate onto the postsynaptic cell, it opens ionotropic glutamate receptor channels. The net flow of current is inward, so a current sink is generated. A nearby electrode (#2) detects this as a negativity. An intracellular electrode placed inside the cell body (#1) records the change in membrane potential that the incoming current causes. Extracellular field potentials are local current sinks or sources that are generated by the collective activity of many cells. Usually a field potential is generated by the simultaneous activation of many neurons by synaptic transmission. The diagram to the right shows hippocampal synaptic field potentials. At the right, the lower trace shows a negative wave that corresponds to a current sink caused by positive charges entering cells through postsynaptic glutamate receptors, while the upper trace shows a positive wave that is generated by the current that leaves the cell (at the cell body) to complete the circuit. For more information, see local field potential. Amperometry Amperometry uses a carbon electrode to record changes in the chemical composition of the oxidized components of a biological solution. Oxidation and reduction is accomplished by changing the voltage at the active surface of the recording electrode in a process known as "scanning". Because certain brain chemicals lose or gain electrons at characteristic voltages, individual species can be identified. Amperometry has been used for studying exocytosis in the neural and endocrine systems. Many monoamine neurotransmitters, e.g., norepinephrine (noradrenalin), dopamine, serotonin (5-HT), are oxidizable. The method can also be used with cells that do not secrete oxidizable neurotransmitters by "loading" them with 5-HT or dopamine. Planar patch clamp Planar patch clamp is a novel method developed for high throughput electrophysiology. Instead of positioning a pipette on an adherent cell, cell suspension is pipetted on a chip containing a microstructured aperture. Schematic drawing of the classical patch clamp configuration. The patch pipette is moved to the cell using a micromanipulator under optical control. Relative movements between the pipette and the cell have to be avoided in order to keep the cell-pipette connection intact. In planar patch configuration the cell is positioned by suction - relative movements between cell and aperture can then be excluded after sealing. An Antivibration table is not necessary. A single cell is then positioned on the hole by suction and a tight connection (Gigaseal) is formed. The planar geometry offers a variety of advantages compared to the classical experiment: - it allows for integration of microfluidics, which enables automatic compound application for ion channel screening. - the system is accessible for optical or scanning probe techniques - perfusion of the intracellular side can be performed. See also Alpha rhythm Auditory evoked potentials Basal skin resistance Bioelectromagnetism Contingent negative variation Cardiac electrophysiology Delta rhythm Electrical activity Electrical brain stimulation Electric fish Electrolyte Electrophysiological techniques for clinical diagnosis Electrophysiology study of heart function Evoked potentials Galvanism Galvanic skin response Kindling Neuroelectrodynamics Olfactory evoked potentials Postactivation evoked potentials Skin electrical properties Skin potential Skin resistance Somatosensory evoked potentials Transcutaneous Electrical Nerve Stimulator Signal transduction Theta rhythm Visual evoked potentials References & Bibliography Key texts Books Papers Additional material Books Papers Google Scholar v·d·e Electroencephalography (EEG) Related tests Event-related potential Electrocorticography (ECoG) Magnetoencephalography (MEG) Somatosensory evoked potential Brainstem auditory evoked potentials Evoked potentials Negativity Bereitschaftspotential ELAN N100 Visual N1 N170 N200 N2pc N400 Contingent negative variation (CNV) Mismatch negativity Positivity C1 & P1 P200 P300 P3a P3b P600 (late positivity) Late positive component Neural oscillations Alpha wave Beta wave Gamma wave Delta wave Theta rhythm K-complex Sleep spindle Sensorimotor rhythm Mu rhythm Topics 10-20 system Difference due to memory (Dm) Oddball paradigm EEGLAB Neurophysiological Biomarker Toolbox (NBT) External links Online textbook EP Lab Digest - Trade Publication for EP Professionals Ion Channel, Biophysics and Electrophysiology Resources External links for planar patch clamp Qultures, Automated Patch Clamp Device description Patch Clamp on a Chip Equipment*Comparison This page uses Creative Commons Licensed content from Wikipedia (view authors).
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